Chemical eluates from ultra-high molecular weight polyethylene and fibroblast proliferation.

نویسندگان

  • Kate Y Wang
  • J Geoffrey Horne
  • Peter A Devane
  • Tracy Wilson
  • John H Miller
چکیده

Although polyethylene wear particles have been implicated in osteolysis and implant loosening, this study is the first to test whether chemical eluates extracted from ultra-high molecular weight polyethylene (UHMWPE) could also be involved in this process. Eluates were prepared from UHMWPE bar stock and examined for their effects on (3)H-thymidine incorporation by human foreskin fibroblasts grown in 96-well culture plates. Low concentrations of eluates stimulated (3)H-thymidine uptake; whereas, high concentrations inhibited uptake in a dose-dependent manner. Maximum inhibition of proliferation for eluates (87+/-0.03% inhibition, n = 45 paired wells) was greater than that observed for particles (54+/-0.07% inhibition, n = 45 paired wells). Ethylene oxide sterilization of UHMWPE reduced (3)H-thymidine uptake at low eluate concentrations relative to sterilization by gamma-irradiation. It was concluded that leachable eluates from UHMWPE implantse contribute to the osteolytic process at the bone-implant interface.

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عنوان ژورنال:
  • Journal of orthopaedic surgery

دوره 9 1  شماره 

صفحات  -

تاریخ انتشار 2001